Unveiling the Mystery of the Epigenetic Aging Clock: A Review of Research on the Impact of Epigenetic Editing at Single Age-Associated CpG Sites on the Genome-wide Epigenetic Aging Landscape I. Research Background and Scientific Questions Epigenetics, especially DNA methylation, has become a cutting-edge frontier in aging mechanism research in rece...

Background Introduction In cell biology, understanding how cells respond differently to perturbations is crucial. Perturbation refers to altering cell states through gene editing, chemical substances, environmental changes, or mechanical forces to study their functions. However, existing methods have limitations in quantifying heterogeneous respons...

The Impact of the Maternal X Chromosome on Cognition and Brain Aging in Female Mice Background Introduction In mammals, female cells possess two X chromosomes, one from the mother (maternal X chromosome, Xm) and one from the father (paternal X chromosome, Xp). During embryonic development, one of the X chromosomes is randomly inactivated, a process...

Genomic Medicine and Re-annotation of CRISPR Guides: Application and Validation of the EXORCISE Algorithm Academic Background The advent of CRISPR-Cas9 technology has revolutionized genetic screening, particularly in studying gene essentiality and chemo-genetic interactions. By designing guide RNAs (gRNAs) that target specific genes, the CRISPR-Cas...

Report on the Academic Paper Paper Source The paper, titled “Modulation of TCR Stimulation and Pifithrin-α Improves the Genomic Safety Profile of CRISPR-Engineered Human T Cells,” was authored by Laurenz T. Ursch, Jule S. Müschen, Julia Ritter, and others from institutions such as the Technical University of Munich and the University of Freiburg Me...