Pathogenic Variants in the Polycystin Pore Helix Cause Distinct Forms of Channel Dysfunction

Medicine, Basic Medical Sciences, Nephrology, Life Sciences, Cell Biology, Biochemistry, Genetics, Biophysics,
autosomal dominant polycystic kidney disease   polycystin   PKD2   ion channels   cryo-EM   channel dysfunction   protein assembly  

Molecular Mechanism Analysis of ADPKD Pathogenic Variants in Ion Channels – In-depth Interpretation of PNAS 2025 Latest Original Research I. Academic Research Background and Scientific Significance Autosomal Dominant Polycystic Kidney Disease (ADPKD) is one of the most common monogenic disorders worldwide, affecting millions of individuals. The pat...

Amortized Template Matching of Molecular Conformations from Cryoelectron Microscopy Images Using Simulation-Based Inference

Information Science, Artificial Intelligence, Medicine, Medical Imaging, Life Sciences, Cell Biology, Biochemistry, Biophysics,
cryo-EM   biophysics   Bayesian inference   molecular modeling   template matching   deep learning   structural biology  

Accelerating Single-Molecule Structure Identification with Simulation-Based Inference — Research Report on “Amortized Template Matching of Molecular Conformations from Cryoelectron Microscopy Images using Simulation-Based Inference” Research Background and Significance In the fields of molecular biology and structural biology, understanding how bio...

Structural Insights into the Ubiquitin-Independent Midnolin-Proteasome Pathway

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Biophysics,
proteasome   ubiquitin-independent degradation   Midnolin   cryo-EM   B-cell malignancies  

Academic Background Protein homeostasis (proteostasis) is a core mechanism for maintaining normal cellular function, with the ubiquitin-proteasome system (UPS) responsible for degrading approximately 80% of abnormal proteins. Traditionally, proteins were thought to require ubiquitination for recognition and degradation by the 26S proteasome. Howeve...

DockEM: An Enhanced Method for Atomic-Scale Protein–Ligand Docking Refinement Leveraging Low-to-Medium Resolution Cryo-EM Density Maps

Chemistry, Medicinal Chemistry, Computational Chemistry, Life Sciences, Biochemistry, Biophysics,
protein-ligand docking   cryo-EM   molecular docking   drug screening   structural biology  

Academic Background and Research Motivation In recent years, protein–ligand docking has rapidly developed as a core technology for virtual drug screening and structure-based drug discovery. Despite improvements in drug discovery efficiency through large-scale high-throughput screening technologies, new drug development still faces high costs, long ...

Overcoming the Preferred-Orientation Problem in Cryo-EM with Self-Supervised Deep Learning

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Biochemistry, Biophysics,
cryo-EM   preferred-orientation problem   self-supervised learning   deep learning   3D reconstruction   molecular structure   biological macromolecules  

Overcoming the Preferred-Orientation Problem in Single-Particle Cryo-EM: An Innovative Solution through Deep Learning Background Introduction In recent years, single-particle cryogenic electron microscopy (Single-Particle Cryo-EM) has become a core technique in structural biology due to its ability to resolve the atomic-resolution structures of bio...