Cancer is a major global public health concern, with its complex pathological processes and diverse manifestations being a focus of research. Many studies have shown that acid-base imbalance plays a crucial role in the occurrence and development of cancer, but the underlying mechanisms are not fully understood. In this study titled “Acid–base homeo...

Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...

Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...

Sequence-based Functional Metagenomics Reveals New Natural Diversity of Functional COPA Genes in Environmental Microbiomes The natural diversity of functional genes/proteins in environmental microbiomes is an essential component of evolutionary and bioengineering research. To gain a deeper understanding of the diversity of the copper (Cu) resistanc...

Research Background Cardiovascular diseases (CVDs) are one of the leading causes of death worldwide, with vascular remodeling and stiffness being critical indicators that significantly affect disease progression and prognosis. Recent studies have increasingly focused on the role of vascular fibroblasts in vascular stiffness and remodeling. In parti...