Intercellular Contractile Force Attenuates Chemosensitivity Through Notch-MVP-Mediated Nuclear Drug Export

Medicine, Oncology, Life Sciences, Cell Biology, Bioengineering, Biophysics,
intercellular force   contractility   cell mechanics   mechanotransduction   chemosensitivity  

Academic Background Chemotherapy resistance is one of the major challenges in cancer treatment. Traditional research has primarily focused on biochemical mechanisms (e.g., drug efflux pumps, DNA repair, etc.), but in recent years, the role of biomechanical factors in tumor progression and drug resistance has gained increasing attention. Studies hav...

Unconventional Secretion of Park7 Requires Lysosomal Delivery via Chaperone-Mediated Autophagy and Specialized SNARE Complex

Medicine, Neurology, Life Sciences, Cell Biology, Biochemistry, Immunology, Biophysics,
unconventional secretion   Park7/DJ-1   autophagy   SNARE complex   oxidative stress  

1. Research Background The Parkinson’s disease-associated protein PARK7/DJ-1 (hereafter referred to as PARK7) is a multifunctional protein that plays critical roles in various pathological conditions including neurodegenerative diseases, cancer, and inflammation. Despite lacking a conventional N-terminal signal peptide sequence, PARK7 can be secret...

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates Using Cryoelectron Tomography

Life Sciences, Cell Biology, Biochemistry, Genetics, Biophysics,
Cryoelectron Tomography   Biomolecular Condensate   Chromatin   Phase Separation   Nucleosome  

Academic Background Biomolecular condensates are membrane-less organelles formed through liquid-liquid phase separation (LLPS) within cells, playing critical roles in key biological processes such as gene expression and signal transduction. However, due to limitations in traditional imaging techniques, high-resolution structural information about t...

DeepRNA-Twist: Language-Model-Guided RNA Torsion Angle Prediction with Attention-Inception Network

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Biochemistry, Bioengineering, Biophysics,
RNA language model   torsion and pseudo-torsion angles   inception network   deep learning   RNA structure prediction   bioinformatics   long-range dependencies  

1. Academic Background and Research Motivation With the rapid development of life sciences and bioinformatics, research into RNA molecular structure and function has become a hot topic. RNA is not merely a carrier of genetic information, but also plays critical roles in regulation, catalysis, and various physiological processes. The three-dimension...

Problem Solving Protocol: Accurate Residue-Level Phase Separation Prediction Using Protein Conformational and Language Model Embeddings

Life Sciences, Biochemistry, Genetics, Biophysics,
phase separation   language model   residue-level prediction   conformational embeddings   intrinsically disordered regions  

1. Academic Background and Research Significance In recent years, protein liquid–liquid phase separation (PS) has emerged as a key mechanism regulating biomolecules inside cells, attracting widespread attention in the life sciences. Phase separation not only drives the formation of biomolecular condensates (membraneless organelles), but also extens...