Molecular Evolution of Protein Sequences and Codon Usage in Monkeypox Viruses

Medicine, Infectious Diseases, Life Sciences, Cell Biology, Biochemistry, Genetics, Microbiology,
mpox virus   positive selection   opg027   accelerated evolution   codon usage bias  

Molecular Evolution of Monkeypox Virus Protein Sequences and Codon Usage Research Background The 2022 Monkeypox virus (MPXV) outbreak has caused significant global public health concern. However, the evolutionary mechanisms of MPXV remain not fully understood. MPXV is a linear double-stranded DNA virus belonging to the family Poxviridae, subfamily ...

Acid-Base Homeostasis and its Implications on Cancer Phenotypic Behaviors

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Bioengineering, Biophysics,
acid-base homeostasis   cancer microenvironment   metabolic reprogramming   Fenton reaction   iron metabolism  

Cancer is a major global public health concern, with its complex pathological processes and diverse manifestations being a focus of research. Many studies have shown that acid-base imbalance plays a crucial role in the occurrence and development of cancer, but the underlying mechanisms are not fully understood. In this study titled “Acid–base homeo...

Grepore-seq: A Robust Workflow to Detect Changes After Gene Editing Through Long-Range PCR and Nanopore Sequencing

Life Sciences, Cell Biology, Biochemistry, Bioengineering, Genetics,
CRISPR/Cas9   genetic change   long-range PCR   nanopore sequencing   grepore-seq  

Grepore-seq: A Robust Workflow for Detecting Gene Editing Changes through Long-Range PCR and Nanopore Sequencing Research Background The CRISPR/Cas9 system, as an RNA-guided DNA endonuclease system, has been widely applied in genome editing. As its potential in clinical treatment continues to increase, comprehensive evaluation of gene editing resul...

Superior Fidelity and Distinct Editing Outcomes of SaCas9 Compared with SpCas9 in Genome Editing

Medicine, Hematology, Life Sciences, Cell Biology, Biochemistry, Bioengineering, Genetics,
genome editing   sacas9   spcas9   PAM sequence   insertion efficiency   off-target effects  

Comparison of SaCas9 and SpCas9 in High-Fidelity and Different Gene Editing Outcomes Research Background CRISPR systems based on Cas9 protein have become powerful tools for genome editing, widely used in basic research and clinical gene therapy. Currently, the most commonly used Cas9 variants are SpCas9 from Streptococcus pyogenes and SaCas9 from S...

Comprehensive Characterization and Global Transcriptome Analysis of Human Fetal Liver Terminal Erythropoiesis

Medicine, Hepatobiliary System, Basic Medical Sciences, Hematology, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
human fetal liver   terminal erythropoiesis   transcriptome   immortalized erythroid cell line   enucleation  

Comprehensive Characterization and Transcriptome Analysis of Terminal Erythropoiesis in Human Fetal Liver Background and Research Question Erythropoiesis is the process of red blood cell production. Initially, “primitive” erythropoiesis occurs in the yolk sac, gradually replaced by “terminal” erythropoiesis in the fetal liver (FL) and postnatal bon...

Decoding Human Biology and Disease Using Single-Cell Omics Technologies

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Genetics,
single-cell omics   computational method   cellular heterogeneity   disease   cancer research  

Decoding Human Biology and Disease with Single-Cell Omics Technologies Background Introduction Cells are the fundamental units of life. A single fertilized egg can develop into an entire complex human body, composed of approximately 37 trillion cells organized into various tissues, organs, and systems. Traditional cell classification methods primar...