In Vivo AAV-SB-CRISPR Screens of Tumor-Infiltrating Primary NK Cells Identify Genetic Checkpoints of CAR-NK Therapy

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Bioengineering, Immunology, Genetics,
AAV-SB-CRISPR screening   Primary NK cells   CAR-NK therapy   Genetic checkpoints   Immunotherapy   Tumor treatment  

AAV-SB-CRISPR Screening Identifies Genetic Checkpoints in Tumor-Infiltrating Primary Natural Killer Cells for CAR-NK Therapy With the rapid advancement of biotechnology and gene editing technologies, increasing attention is being paid to enhancing the clinical potential of natural killer (NK) cells in cancer therapy. Despite their potential in clin...

Mapping Variant Effects on Anti-tumor Hallmarks of Primary Human T Cells with Base-Editing Screens

Mapping Variant Effects on Anti-tumor Hallmarks of Primary Human T Cells with Base-Editing Screens

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Genetics,
base editing   T cells   cancer immunotherapy   single-nucleotide variants   gain-of-function   loss-of-function   immune cell therapy  

Application of Base Editing Screening Technology in Tumor Immunotherapy T Cell Research Immune cell therapies, such as T cell transfer therapy and CAR T cell therapy, have become important clinical cancer treatment tools in certain disease fields. However, most cancer patients fail to benefit from cell therapies, possibly due to inherent changes in...

Tracking-seq Reveals the Heterogeneity of Off-target Effects in CRISPR-Cas9-mediated Genome Editing

Medicine, Basic Medical Sciences, Life Sciences, Immunology, Genetics, Biophysics, Microbiology,
CRISPR-Cas9   off-target effects   genome editing   Tracking-seq   genome editing tools  

Transcriptomics Technology Tracking-Seq Reveals Off-Target Effect Heterogeneity Mediated by CRISPR-Cas9 Research Background With the rapid development of genome editing technologies, the CRISPR-Cas9 system has been widely used in biomedical research due to its high efficiency and ease of operation. However, a significant issue with the CRISPR-Cas9 ...

Single-Cell Analysis of Anti-BCMA CAR T Cell Therapy in Patients with Central Nervous System Autoimmunity

Medicine, Rheumatology and Immunology, Neurology, Life Sciences, Cell Biology, Immunology,
BCMA   CAR T cells   central nervous system   autoimmune diseases   single-cell analysis   neuroinflammation  

Central Nervous System (CNS) autoimmune diseases, such as Neuromyelitis Optica Spectrum Disorders (NMOSD), are inflammatory demyelinating conditions characterized by relapses of optic neuritis and myelitis. Despite significant progress in the treatment of NMOSD, a subset of patients responds poorly to current therapies, with recurrent and difficult...

Oxidative Phosphorylation Regulates B Cell Effector Cytokines and Promotes Inflammation in Multiple Sclerosis

Medicine, Rheumatology and Immunology, Neurology, Life Sciences, Cell Biology, Biochemistry, Immunology,
oxidative phosphorylation   B cells   cytokines   inflammation   multiple sclerosis   metabolism  

Oxidative Phosphorylation Regulates B Cell Effector Cytokines and Promotes Inflammation in Multiple Sclerosis Background Introduction In recent years, the antibody-independent functions of B cells in health and disease have become a research hotspot, particularly their ability to secrete different cytokines that can activate or downregulate local i...

Nucleobase Adducts Bind MR1 and Stimulate MR1-Restricted T Cells

Medicine, Rheumatology and Immunology, Medical Laboratory Science, Gastroenterology, Oncology, Life Sciences, Cell Biology, Immunology,
nucleobase adducts   mr1   t cells   tumor cells   carbonyl stress   metabolic changes   antigens  

Nucleotide Base Adducts Bind MR1 and Stimulate MR1-Restricted T Cells Abstract This article provides an overview of the latest research by Vacchini et al. on MR1-restricted T cells (MR1T cells), which discovered the presence of nucleotide base adducts as MR1T cell antigens and unveiled their metabolic pathways and physiological functions in tumor c...