Tracking-seq Reveals the Heterogeneity of Off-target Effects in CRISPR-Cas9-mediated Genome Editing

Medicine, Basic Medical Sciences, Life Sciences, Immunology, Genetics, Biophysics, Microbiology,
CRISPR-Cas9   off-target effects   genome editing   Tracking-seq   genome editing tools  

Transcriptomics Technology Tracking-Seq Reveals Off-Target Effect Heterogeneity Mediated by CRISPR-Cas9 Research Background With the rapid development of genome editing technologies, the CRISPR-Cas9 system has been widely used in biomedical research due to its high efficiency and ease of operation. However, a significant issue with the CRISPR-Cas9 ...

Strain Tracking in Complex Microbiomes Using Synteny Analysis Reveals Per-Species Modes of Evolution

Life Sciences, Cell Biology, Genetics, Microbiology,
synteny analysis   strain tracking   microbiomes   structural changes   point mutations  

Using Genome Synteny Analysis for Strain Tracking in Complex Microbiomes Reveals Species-Specific Evolutionary Patterns Background Microbial populations differentiate into distinct strains through single-nucleotide mutations and structural variations such as recombination, insertions, and deletions. Most strain comparison methods primarily quantify...

Crowd-sourced Benchmarking of Single-sample Tumor Subclonal Reconstruction

Information Science, Artificial Intelligence, Medicine, Medical Laboratory Science, Oncology, Life Sciences, Cell Biology, Genetics,
tumor subclonal reconstruction   tumor evolution   single-sample analysis   crowd-sourced benchmarking   DNA sequencing  

Single-Sample Tumor Subclonal Reconstruction Algorithm Based on Crowd-Sourced Resources Background The evolution of cancer and the genetic heterogeneity of tumors are critical fields in modern oncology research. Tumors evolve from normal cells through progressive acquisition of somatic mutations. These mutations occur probabilistically, influenced ...

Graph Neural Networks with Multiple Prior Knowledge for Multi-omics Data Analysis

Graph Neural Networks with Multiple Prior Knowledge for Multi-omics Data Analysis

Information Science, Computer Science, Artificial Intelligence, Medicine, Environmental and Public Health, Oncology, Life Sciences, Cell Biology, Genetics,
graph neural networks   multi-omics data   contrastive learning   biological knowledge   cancer molecular classification  

Multiple Prior Knowledge Graph Neural Network in Multi-Omics Data Analysis Background Introduction Precision medicine is an important field for the future of healthcare as it provides personalized treatment plans for patients, improving treatment outcomes and reducing costs. For instance, due to the complex clinical, pathological, and molecular cha...

Single-Subject Cortical Morphological Brain Networks: Phenotypic Associations and Neurobiological Substrates

Medicine, Basic Medical Sciences, Neurology, Medical Imaging, Life Sciences, Cell Biology, Biochemistry, Immunology, Genetics, Biophysics,
morphological brain network   structural magnetic resonance imaging   cortical thickness   sex   neurotransmitter  

This paper is a study on the phenotypic associations and neurobiological bases of single-subject morphological brain networks. Combining multimodal and multiscale data, this study reveals the differences in morphological brain networks between genders, their potential as individual-specific markers, and their relationships with gene expression, lay...

Associations of Epigenetic Age Estimators with Cognitive Function Trajectories in the Women's Health Initiative Memory Study

Medicine, Geriatrics, Neurology, Life Sciences, Biochemistry, Genetics,
epigenetics   age estimation   cognitive function   women's health   memory  

Association Between Epigenetic Age Acceleration Estimates and Cognitive Trajectories in the Women’s Health Initiative Memory Study Background Maintaining cognitive function is crucial for independent living among older adults. With the growth of the aging population, cognitive decline has become a significant public health challenge. Many studies h...