Bidirectional Epigenetic Editing Reveals Hierarchies in Gene Regulation Background and Research Motivation In the human genome, the role of non-coding elements such as enhancers in gene regulation is widely recognized. However, current CRISPR interference methods still have some limitations in studying non-coding elements and genetic interactions. ...

Cerebrospinal Fluid Proteomics Study for Diagnosis and Prediction of Alzheimer’s Disease Background and Research Objectives Alzheimer’s disease (AD) is a neurodegenerative disease that leads to memory loss and cognitive decline, and currently, there is no effective cure globally. Traditionally, the pathological features of AD include β-amyloid (Aβ)...

Academic Background In the field of plant biotechnology, traditional gene manipulation methods focus on producing the desired phenotypes and cellular activities through continuous transgene expression. However, strong continuous promoters may lead to gene silencing, metabolic burdens, or other adverse effects on yield, preventing the full realizati...

Generation of Clinically Applicable CAR-NKT Cells Research Background Chimeric Antigen Receptor (CAR) T cell therapy has been approved by the Food and Drug Administration (FDA) for the treatment of B-cell malignancies and multiple myeloma. However, the use of autologous CAR-T cell products presents challenges such as high costs, long manufacturing ...

Breakthrough in Long-term Live Subcellular Imaging: Study of Confocal Scanning Light-Field Microscopy Research Background Long-term live cell dynamic observation is indispensable in studying physiological pathological processes such as immune response and brain function, requiring high spatiotemporal resolution and low phototoxicity. Existing confo...

Academic Background RNA isoforms play a critical role in gene expression regulation. On average, human protein-coding genes contain over eight RNA isoforms, leading to nearly four distinct protein-coding sequences. Traditional short-read sequencing technologies, due to their limitations in read length, cannot accurately assemble and quantify RNA is...