Single-Molecule Dynamic Structural Biology with Vertically Arranged DNA on a Fluorescence Microscope

Chemistry, Nanoscience, Life Sciences, Biochemistry, Biophysics,
single-molecule fluorescence   DNA-protein interactions   graphene energy transfer   dynamic structural biology   super-resolution microscopy  

Single-Molecule Dynamic Structural Biology: Breakthrough in Observing DNA-Protein Interactions Using Graphene Background Introduction The intricate and precise interactions between DNA and proteins play a crucial role in fundamental biological functions such as DNA replication, transcription, and repair. However, observing these interaction process...

EvoAI Enables Extreme Compression and Reconstruction of the Protein Sequence Space

Information Science, Artificial Intelligence, Life Sciences, Biochemistry, Bioengineering, Genetics,
protein engineering   deep learning   sequence space compression   high-dimensional space   biomolecular design  

Extreme Compression and Reconstruction of Protein Sequence Space: A Breakthrough Study on EvoAI Background Protein design and optimization have become central challenges in fields like biotechnology, medicine, and synthetic biology. The functions of proteins are determined by their sequences and structures, but this functional sequence space is hig...

Evaluation of Large Language Models for Discovery of Gene Set Function

Information Science, Artificial Intelligence, Medicine, Life Sciences, Biochemistry, Genetics,
Large Language Models   Gene Set Function   Functional Genomics   Gene Ontology   Bioinformatics   Artificial Intelligence   Gene Expression  

Exploration of Gene Set Function Discovery Using Large Language Models: GPT-4 Excels Academic Background In functional genomics, gene set enrichment analysis is a critical methodology for understanding gene functions and their associated biological processes. However, existing enrichment analyses rely heavily on curated gene function databases, suc...

Overcoming the Preferred-Orientation Problem in Cryo-EM with Self-Supervised Deep Learning

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Biochemistry, Biophysics,
cryo-EM   preferred-orientation problem   self-supervised learning   deep learning   3D reconstruction   molecular structure   biological macromolecules  

Overcoming the Preferred-Orientation Problem in Single-Particle Cryo-EM: An Innovative Solution through Deep Learning Background Introduction In recent years, single-particle cryogenic electron microscopy (Single-Particle Cryo-EM) has become a core technique in structural biology due to its ability to resolve the atomic-resolution structures of bio...

Direct Observation of Small Molecule Activator Binding to Single PR65 Protein

Chemistry, Medicinal Chemistry, Medicine, Life Sciences, Biochemistry, Biophysics,
Protein Phosphatase 2A   Small Molecule Activator   Nanoaperture Optical Tweezers   Binding Kinetics   Molecular Dynamics Simulation  

Single-Molecule Optical Tweezer Technique Reveals the Binding Mechanism of a Small Molecule Activator and PR65 Protein Academic Background Protein phosphatase 2A (PP2A) is a crucial enzyme in regulating cellular signaling. Its dysfunction is closely associated with various cancers and chronic diseases, such as Alzheimer’s disease and chronic obstru...

The Development and Application of an Engineered Direct Electron Transfer Enzyme for Continuous Levodopa Monitoring

Chemistry, Analytical Chemistry, Medicinal Chemistry, Medicine, Neurology, Life Sciences, Biochemistry, Bioengineering,
levodopa   Parkinson's disease   direct electron transfer enzyme   biosensor   continuous monitoring  

Development of an Engineered Direct Electron Transfer Enzyme for Continuous Levodopa Monitoring Background Parkinson’s Disease (PD) is a chronic neurodegenerative disease that affects millions of people worldwide. It is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of α-synuclein aggregations forming Lew...