VCP's Nuclear Journey: Initiated by Interacting with KPNB1 to Repair DNA Damage

Chemistry, Medicinal Chemistry, Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Genetics,
DNA damage repair   VCP   KPNB1   nuclear translocation   Withaferin A  

Academic Background DNA damage repair (DDR) is a core mechanism for maintaining genome stability, and its dysfunction is closely associated with cancer development. Valosin-containing protein (VCP/p97), a member of the AAA+ ATPase family, plays a critical role in DDR by recognizing ubiquitinated proteins and recruiting repair factors (e.g., 53BP1, ...

Improved Synapsis Dynamics Accompany Meiotic Stability in Arabidopsis Arenosa Autotetraploids

Life Sciences, Cell Biology, Genetics, Botany,
polyploidy   meiosis   synapsis dynamics   chromosome pairing   crossover   genome duplication   evolution  

1. Research Background Meiosis is the core process of sexual reproduction in eukaryotes, generating haploid gametes through homologous chromosome pairing, synapsis, and crossover. Polyploidy is a major driver of plant evolution, but additional chromosome copies disrupt key meiotic steps, leading to sterility or genomic instability. Core scientific ...

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates Using Cryoelectron Tomography

Life Sciences, Cell Biology, Biochemistry, Genetics, Biophysics,
Cryoelectron Tomography   Biomolecular Condensate   Chromatin   Phase Separation   Nucleosome  

Academic Background Biomolecular condensates are membrane-less organelles formed through liquid-liquid phase separation (LLPS) within cells, playing critical roles in key biological processes such as gene expression and signal transduction. However, due to limitations in traditional imaging techniques, high-resolution structural information about t...

Problem Solving Protocol: Accurate Residue-Level Phase Separation Prediction Using Protein Conformational and Language Model Embeddings

Life Sciences, Biochemistry, Genetics, Biophysics,
phase separation   language model   residue-level prediction   conformational embeddings   intrinsically disordered regions  

1. Academic Background and Research Significance In recent years, protein liquid–liquid phase separation (PS) has emerged as a key mechanism regulating biomolecules inside cells, attracting widespread attention in the life sciences. Phase separation not only drives the formation of biomolecular condensates (membraneless organelles), but also extens...

Chrombus-XMBD: A Graph Convolution Model Predicting 3D-Genome from Chromatin Features

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Cell Biology, Genetics, Biophysics,
3D-genome   graph convolution model   chromatin features   long-range interaction   model generalizability  

Research Background and Disciplinary Significance In eukaryotic cells, the three-dimensional (3D) spatial structure of chromatin plays a crucial role in gene expression regulation. Through complex folding, looping, and local spatial reconfiguration of DNA, different genetic elements (such as promoters and enhancers) are brought into spatial proximi...

Benchmarking Copy Number Aberrations Inference Tools Using Single-Cell Multi-Omics Datasets

Information Science, Artificial Intelligence, Medicine, Oncology, Life Sciences, Cell Biology, Bioengineering, Genetics,
Single-cell RNA sequencing   Copy number aberrations   Tumor heterogeneity   Benchmarking   Multi-omics  

1. Research Background and Significance In the fields of oncology and genomics, chromosomal copy number alterations (CNAs) are a key type of genetic variation driving the occurrence and progression of cancer. CNAs not only determine tumor heterogeneity but also play a crucial role in early tumor detection, subclone evolution analysis, research on d...