Overcoming the Preferred-Orientation Problem in Cryo-EM with Self-Supervised Deep Learning

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Biochemistry, Biophysics,
cryo-EM   preferred-orientation problem   self-supervised learning   deep learning   3D reconstruction   molecular structure   biological macromolecules  

Overcoming the Preferred-Orientation Problem in Single-Particle Cryo-EM: An Innovative Solution through Deep Learning Background Introduction In recent years, single-particle cryogenic electron microscopy (Single-Particle Cryo-EM) has become a core technique in structural biology due to its ability to resolve the atomic-resolution structures of bio...

Fully Automated Sample-to-Result Simple-RPA Microfluidic Chip for In Ovo Sexing Application

Chemistry, Analytical Chemistry, Engineering, Mechanical Engineering, Medicine, Life Sciences, Bioengineering,
microfluidic chip   in ovo sexing   recombinase polymerase amplification   automated detection   low cost   high sensitivity   animal welfare  

Fully Automated Microfluidic Chip-Based Embryo Sexing Technology Academic Background In the egg-laying poultry industry, newly hatched male chicks are often culled immediately after hatching, as they neither lay eggs nor produce high-quality meat. Each year, approximately 372 million male chicks are culled in the European Union. This practice has s...

Direct Observation of Small Molecule Activator Binding to Single PR65 Protein

Chemistry, Medicinal Chemistry, Medicine, Life Sciences, Biochemistry, Biophysics,
Protein Phosphatase 2A   Small Molecule Activator   Nanoaperture Optical Tweezers   Binding Kinetics   Molecular Dynamics Simulation  

Single-Molecule Optical Tweezer Technique Reveals the Binding Mechanism of a Small Molecule Activator and PR65 Protein Academic Background Protein phosphatase 2A (PP2A) is a crucial enzyme in regulating cellular signaling. Its dysfunction is closely associated with various cancers and chronic diseases, such as Alzheimer’s disease and chronic obstru...

The Development and Application of an Engineered Direct Electron Transfer Enzyme for Continuous Levodopa Monitoring

Chemistry, Analytical Chemistry, Medicinal Chemistry, Medicine, Neurology, Life Sciences, Biochemistry, Bioengineering,
levodopa   Parkinson's disease   direct electron transfer enzyme   biosensor   continuous monitoring  

Development of an Engineered Direct Electron Transfer Enzyme for Continuous Levodopa Monitoring Background Parkinson’s Disease (PD) is a chronic neurodegenerative disease that affects millions of people worldwide. It is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of α-synuclein aggregations forming Lew...

Advancements of Paper-Based Sensors for Antibiotic-Resistant Bacterial Species Identification

Chemistry, Nanoscience, Medicine, Infectious Diseases, Life Sciences, Biochemistry, Microbiology,
paper-based sensors   antibiotic resistance   bacterial infections   nanoparticles   detection techniques  

Advances in Paper-Based Sensors for the Detection of Antibiotic-Resistant Bacteria Background Antimicrobial resistance (AMR) is one of the most significant global public health challenges today. With the extensive use and misuse of antibiotics, more and more bacteria have developed resistance, rendering traditional treatments ineffective. According...