Triple Knockdown of CD11a, CD49d, and PSGL1 in T Cells Reduces CAR-T Cell Toxicity but Preserves Activity Against Solid Tumors in Mice

Medicine, Oncology, Life Sciences, Cell Biology, Immunology,
CAR-T cells   solid tumors   immunotherapy   cell adhesion   cell migration  

Study on Reducing Toxicity of CAR-T Cell Therapy for Solid Tumors Academic Background Chimeric antigen receptor T-cell (CAR-T) therapy has made significant progress in treating hematologic malignancies, but its application in solid tumors faces major challenges. Solid tumors often lack tumor-specific antigens (TSAs), and CAR-T cells may attack norm...

Ciliopathies: Undocking of an Extensive Ciliary Network Induces Proteostasis and Cell Fate Switching Resulting in Severe Primary Ciliary Dyskinesia

Medicine, Respiratory System, Life Sciences, Cell Biology, Biochemistry, Genetics,
Primary Ciliary Dyskinesia   Ciliary Network   Proteostasis   Cell Fate Switching   Gene Therapy  

Research on Ciliary Diseases: The Role of CCDC39/CCDC40 Heterodimer in Primary Ciliary Dyskinesia Academic Background Primary Ciliary Dyskinesia (PCD) is a rare monogenic disorder characterized by chronic respiratory infections, infertility, and organ laterality defects. Although more than 50 genes associated with PCD have been identified, mutation...

Selective Abrogation of S6K2 Identifies Lipid Homeostasis as a Survival Vulnerability in MAPK Inhibitor–Resistant NRAS-Mutant Melanoma

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
NRAS-mutant melanoma   MAPK inhibitor resistance   lipid metabolism   S6K2   PPARα  

Academic Background NRAS-mutant (NRASmut) melanoma is a highly aggressive tumor type, accounting for nearly 30% of all melanoma cases. NRAS is an oncogene that persistently activates the mitogen-activated protein kinase (MAPK) signaling pathway, which plays a critical role in melanoma development. However, despite extensive research on MAPK pathway...

Age-Dependent Macropinocytosis Drives Resistance to KRAS-G12D–Targeted Therapy in Advanced Pancreatic Cancer

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
Pancreatic Cancer   KRAS-G12D   Macropinocytosis   Drug Resistance   Targeted Therapy  

Academic Background and Problem Pancreatic Ductal Adenocarcinoma (PDAC) is an aggressive cancer with an extremely low five-year survival rate, primarily due to late diagnosis and limited treatment options. Approximately 95% of PDAC patients harbor KRAS mutations, with the KRAS-G12D mutation being the most common. Although KRAS mutations have long b...

High-Throughput Discovery of Inhibitory Protein Fragments with AlphaFold

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Cell Biology, Biochemistry, Bioengineering, Biophysics,
inhibitory peptides   protein interactions   deep mutational scanning   high-throughput computation   FragFold  

High-Precision Prediction of Protein Fragment Inhibitory Activity: The Application of FragFold Academic Background Protein interactions play a crucial role in cellular life activities, and peptides or protein fragments can regulate protein functions by binding to specific protein interfaces, even acting as inhibitors. Recent developments in high-th...

Biochemical and Structural Bases for Talin ABSS–F-Actin Interactions

Life Sciences, Cell Biology, Biochemistry, Bioengineering, Biophysics,
Focal Adhesion   Cryo-EM   Actin   Talin   Mechanobiology  

Academic Background In cell biology, focal adhesions (FAs) are key connection points between cells and the extracellular matrix (ECM), linking integrin receptors to the intracellular actin cytoskeleton. They play a crucial role in cell migration and polarization. Talin is a core protein in focal adhesions that directly connects integrin receptors t...