CRISPR-dCas9 Activation of TSG-6 in MSCs Modulates the Cargo of MSC-Derived Extracellular Vesicles and Attenuates Inflammatory Responses in Human Intervertebral Disc Cells In Vitro

Medicine, Rheumatology and Immunology, Orthopedics, Life Sciences, Cell Biology, Immunology,
CRISPR-dCas9   TSG-6   Mesenchymal Stem Cells   Extracellular Vesicles   Intervertebral Disc Cells   Inflammatory Responses   Genomic Engineering  

Intervertebral Disc Degeneration (IVDD) is one of the leading causes of low back pain worldwide, significantly impacting patients’ quality of life. IVDD is often accompanied by inflammatory responses, leading to the degradation of the extracellular matrix (ECM) and structural damage. Although cell therapies, such as mesenchymal stem cells (MSCs), h...

Empowering High Throughput Screening of 3D Models: Automated Dispensing of Cervical and Endometrial Cancer Cells

Medicine, Obstetrics and Gynecology, Oncology, Life Sciences, Cell Biology, Bioengineering,
High Throughput Screening   3D Printing   Tissue Engineering   Biofabrication   Cancer Engineering  

Cervical cancer and endometrial cancer are significant challenges in women’s healthcare, with high mortality rates and limited treatment options making related research particularly important. Traditional two-dimensional (2D) cell screening models can provide information on the effects of drugs on single cells but fail to capture multicellular inte...

Obesity-Associated Conditions Hinder Solute Drainage Function of Engineered Human Lymphatic Vessels

Medicine, Life Sciences, Cell Biology, Bioengineering,
obesity   lymphatic vessels   solute drainage   inflammation   hypoxia   hyperlipidemia   microenvironment  

Obesity is a growing global health issue, closely linked to metabolic diseases such as cardiovascular diseases and diabetes, as well as lymphatic dysfunction. The lymphatic system plays a crucial role in maintaining fluid balance, immune responses, and fat metabolism. However, lymphatic function is often impaired in obese patients, leading to compl...

Theoretical and Experimental Studies of the Dynamic Damage of Endothelial Cellular Networks under Ultrasound Cavitation

Medicine, Life Sciences, Cell Biology, Bioengineering,
endothelial cells   intercellular junctions   cell-cell rupture   cavitation damage   ultrasound cavitation  

Endothelial cells play a crucial role in the vascular system, regulating hemostasis, vasodilation, immune responses, and inflammation through intercellular junctions (IJs). However, excessive mechanical loading can lead to endothelial cell damage and endothelial barrier dysfunction. Understanding the dynamic rupture mechanisms of intercellular junc...

Hyperosmotic Stress Promotes the Nuclear Translocation of TFEB in Tubular Epithelial Cells Depending on Intracellular Ca2+ Signals via TRPML Channels

Medicine, Urology, Nephrology, Life Sciences, Cell Biology, Biochemistry, Bioengineering,
Hyperosmolarity   Transcription Factor EB (TFEB)   Calcineurin   TRPML1   Tubular Epithelial Cell  

In recent years, autophagy, as a crucial intracellular degradation and recycling mechanism, has played a key role in maintaining cellular homeostasis and responding to various stress conditions. Particularly in renal proximal tubular epithelial cells, autophagy activity is essential for coping with common renal injuries such as ischemia, toxic dama...

Matrix Stiffness-Mediated DNA Methylation in Endothelial Cells

Medicine, Oncology, Life Sciences, Cell Biology, Bioengineering,
DNA methylation   endothelial cells   matrix stiffness   DNMT1   tumor microenvironment  

In pathological conditions, alterations in tissue mechanical properties are one of the prominent features of many diseases, such as cancer. The tumor vascular system plays a critical role in tumor growth, but its structure and function often become abnormal, manifesting as disorganized, tortuous, and leaky blood vessels. Research has shown that the...