Integrated Analyses of Multi-Omic Data Reveal Metabolic Vulnerability as a Novel Therapeutic Target in Lung Cancer Brain Metastases

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Genetics,
Lung cancer brain metastases   Metabolic vulnerability   Tumor immune microenvironment   Multi-omic analysis   Mitochondrial metabolism  

Report on the Multi-Omics Study of Lung Cancer Brain Metastases (LC-BMs) Academic Background Lung cancer is one of the most prevalent and deadly cancers worldwide. Lung cancer brain metastases (LC-BMs) are a common complication in patients with lung cancer and are associated with poor prognosis. Despite advances in lung cancer therapies, the standa...

Genome-aware annotation of CRISPR guides validates targets in variant cell lines and enhances discovery in screens

Medicine, Oncology, Life Sciences, Cell Biology, Bioengineering, Genetics,
CRISPR   genome annotation   bioinformatics   computational biology   software tool  

Genomic Medicine and Re-annotation of CRISPR Guides: Application and Validation of the EXORCISE Algorithm Academic Background The advent of CRISPR-Cas9 technology has revolutionized genetic screening, particularly in studying gene essentiality and chemo-genetic interactions. By designing guide RNAs (gRNAs) that target specific genes, the CRISPR-Cas...

CD30 Influences Germinal Center B-Cell Dynamics and the Expansion of IgG1-Switched B Cells

Medicine, Rheumatology and Immunology, Life Sciences, Cell Biology, Biochemistry, Immunology,
B lymphocytes   germinal center reaction   CD30   conditional mice   CD30L   senescence-associated T cells  

The Impact of CD30 on Germinal Center B Cell Dynamics and the Expansion of IgG1-Switched B Cells Background CD30 (also known as TNFRSF8) is a member of the tumor necrosis factor receptor (TNF-R) superfamily. Initially identified as a marker of Hodgkin lymphoma, subsequent research revealed its expression on other lymphomas, such as diffuse large B-...

Critical and Differential Roles of eIF4A1 and eIF4A2 in B-cell Development and Function

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
RNA helicase   eIF4A   ribosome biogenesis   translation   B cell  

Critical and Differential Roles of eIF4A1 and eIF4A2 in B-Cell Development and Function Academic Background In mammalian cells, translation initiation is a crucial step in protein synthesis, where the eukaryotic initiation factor 4A (eIF4A) plays a pivotal role. eIF4A is an ATP-dependent RNA helicase that unwinds secondary structures in the 5’ untr...

Mechanisms of Age-Associated Variation in Immune Cell Regeneration Across Individuals

Medicine, Geriatrics, Hematology, Life Sciences, Cell Biology, Immunology,
aging   hematopoietic stem cells   clonal tracking   lineage bias   myelopoiesis  

The functional decline of the immune system with aging is characterized by an increase in innate immune cells (e.g., granulocytes) and a decrease in adaptive immune cells (e.g., B cells). This imbalance in the immune system is closely related to the onset of various diseases, particularly myeloid leukemia and immune deficiencies common in the elder...

Mapping In Silico Genetic Networks of the KMT2D Tumour Suppressor Gene to Uncover Novel Functional Associations and Cancer Cell Vulnerabilities

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Genetics,
tumour suppressor genes   KMT2D   genetic networks   synthetic lethality   WRN inhibitors  

Academic Background and Problem Statement Loss-of-Function (LOF) mutations in Tumor Suppressor Genes (TSGs) are prevalent in cancer. However, therapeutic strategies directly targeting these mutations are challenging due to the loss or reduction of encoded protein function, which in normal states plays a role in inhibiting aberrant cell proliferatio...