Academic Background and Problem Statement Mitochondria, as crucial organelles in eukaryotic cells, are involved in cellular energy metabolism, signal transduction, and the regulation of cell survival and death. Mitochondrial dysfunction is associated with various human diseases, including neurodegenerative disorders, cardiovascular diseases, diabet...
Uranium-Free KMnO₄/Pb Staining Enables Imaging of Tissue Architectures in Low Vacuum Scanning Electron Microscopy Academic Background Electron Microscopy (EM) is one of the most powerful tools for studying the ultrastructure of cells and tissues. However, traditional metal staining methods for biological samples often require the use of harmful ura...
Nucleosome Fibre Topology Guides Transcription Factor Binding to Enhancers Academic Background The establishment of cellular identity relies on the concerted action of multiple transcription factors (TFs) bound to enhancers of cell-type-specific genes. Although TFs recognize specific DNA motifs within accessible chromatin, this information alone is...
Spatial Transcriptomic Clocks Reveal Cell Proximity Effects in Brain Aging Academic Background With aging, cognitive function declines, and the risk of neurodegenerative diseases significantly increases. Brain aging is a complex process accompanied by numerous cellular changes. However, how aging cells affect neighboring cells and how this contribu...
Academic Background and Problem Statement In cancer research, gene amplification is a common form of mutation, particularly in the activation of oncogenes. However, despite the widely recognized importance of gene amplification in cancer, precisely modeling these amplifications in primary cells and model organisms remains a challenge. Specifically,...
In Vitro Model of Vertebrate Trunk Development Academic Background The development of the vertebrate trunk is a highly coordinated process involving the generation and organization of multiple cell types. At the core of this process are progenitor cells located in the posterior of the embryo, which differentiate into tissues such as the neural tube...