Discovery of Large Extracellular Vesicles Blebbisomes and Their Functional Study

Medicine, Oncology, Life Sciences, Cell Biology, Immunology,
extracellular vesicles   Blebbisomes   intercellular communication   mitochondria   immune checkpoint proteins  

Academic Background Extracellular vesicles (EVs) are crucial mediators of intercellular communication, carrying proteins, lipids, and genetic information to participate in various physiological and pathological processes. However, our understanding of EVs remains incomplete, particularly regarding the study of exceptionally large EVs. To address th...

Growth Factor-Triggered De-Sialylation Controls Glycolipid-Lectin-Driven Endocytosis

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
de-sialylation   glycolipid-lectin   endocytosis   cell migration   cell adhesion   glycosylation   epidermal growth factor  

In cell biology, the glycosylation modification of cell surface glycoproteins plays a crucial role in processes such as cell signaling, adhesion, and migration. How dynamic changes in glycosylation regulate intracellular transport and function, particularly the internalization of cell surface receptors through endocytosis, remains an incompletely u...

Intratumour Oxidative Hotspots Provide a Niche for Cancer Cell Dissemination

Medicine, Oncology, Life Sciences, Cell Biology, Immunology,
Intratumour heterogeneity   Oxidative stress   Cancer cell dissemination   Microenvironment   p38-Myc axis  

Tumor heterogeneity is a critical issue in cancer research, encompassing genetic, phenotypic, and microenvironmental heterogeneity. Although single-cell sequencing has shed light on genetic and phenotypic heterogeneity, the study of non-genetic factors, such as microenvironmental heterogeneity, remains insufficient. Oxidative stress is a hallmark o...

Systematic Reconstruction of Molecular Pathway Signatures Using Scalable Single-Cell Perturbation Screens

Medicine, Life Sciences, Cell Biology, Bioengineering, Immunology, Genetics,
single-cell sequencing   gene regulatory networks   CRISPR interference   functional genomics   signaling pathways  

In the field of functional genomics, researchers have long been dedicated to predicting causal regulatory relationships from observational data. However, despite modern technologies enabling the measurement of diverse molecular modalities, inferring causal regulatory relationships from observational data remains challenging. In particular, the iden...

CRISPR-dCas9 Activation of TSG-6 in MSCs Modulates the Cargo of MSC-Derived Extracellular Vesicles and Attenuates Inflammatory Responses in Human Intervertebral Disc Cells In Vitro

Medicine, Rheumatology and Immunology, Orthopedics, Life Sciences, Cell Biology, Immunology,
CRISPR-dCas9   TSG-6   Mesenchymal Stem Cells   Extracellular Vesicles   Intervertebral Disc Cells   Inflammatory Responses   Genomic Engineering  

Intervertebral Disc Degeneration (IVDD) is one of the leading causes of low back pain worldwide, significantly impacting patients’ quality of life. IVDD is often accompanied by inflammatory responses, leading to the degradation of the extracellular matrix (ECM) and structural damage. Although cell therapies, such as mesenchymal stem cells (MSCs), h...

Microbiome Dysbiosis, Neutrophil Recruitment and Mesenchymal Transition of Mesothelial Cells Promotes Peritoneal Metastasis of Colorectal Cancer

Medicine, Gastroenterology, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology,
microbiome dysbiosis   neutrophil recruitment   mesothelial cells   mesenchymal transition   colorectal cancer   peritoneal metastasis   tumor microenvironment  

Background Introduction Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide, with high incidence and mortality rates. According to statistics, approximately 1.87 million new cases of CRC were diagnosed globally in 2020, resulting in 915,000 deaths. Peritoneal metastasis (PM) is a common metastatic pathway of CRC, with about ...