Structural Insights into the Ubiquitin-Independent Midnolin-Proteasome Pathway

Medicine, Oncology, Life Sciences, Cell Biology, Biochemistry, Immunology, Biophysics,
proteasome   ubiquitin-independent degradation   Midnolin   cryo-EM   B-cell malignancies  

Academic Background Protein homeostasis (proteostasis) is a core mechanism for maintaining normal cellular function, with the ubiquitin-proteasome system (UPS) responsible for degrading approximately 80% of abnormal proteins. Traditionally, proteins were thought to require ubiquitination for recognition and degradation by the 26S proteasome. Howeve...

Unconventional Secretion of Park7 Requires Lysosomal Delivery via Chaperone-Mediated Autophagy and Specialized SNARE Complex

Medicine, Neurology, Life Sciences, Cell Biology, Biochemistry, Immunology, Biophysics,
unconventional secretion   Park7/DJ-1   autophagy   SNARE complex   oxidative stress  

1. Research Background The Parkinson’s disease-associated protein PARK7/DJ-1 (hereafter referred to as PARK7) is a multifunctional protein that plays critical roles in various pathological conditions including neurodegenerative diseases, cancer, and inflammation. Despite lacking a conventional N-terminal signal peptide sequence, PARK7 can be secret...

METTL3 Mediates Atheroprone Flow–Induced Glycolysis in Endothelial Cells

Medicine, Cardiovascular System, Life Sciences, Cell Biology, Biochemistry, Immunology,
glycolysis   endothelial cells   atherosclerosis   METTL3   flow   RNA modification   metabolic reprogramming  

1. Research Background Atherosclerosis is the primary pathological basis of cardiovascular diseases, closely associated with endothelial cell (EC) dysfunction. Hemodynamic factors play a critical role in the regional selectivity of atherosclerosis: oscillatory shear stress (OS) (e.g., at vascular bifurcations) promotes plaque formation, while pulsa...

Deep scSTAR: Leveraging Deep Learning for the Extraction and Enhancement of Phenotype-Associated Features from Single-Cell RNA Sequencing and Spatial Transcriptomics Data

Information Science, Artificial Intelligence, Medicine, Life Sciences, Cell Biology, Immunology,
Single-cell RNA sequencing   Spatial transcriptomics   Deep learning   Phenotype-associated features   Tumor microenvironment  

In recent years, cutting-edge technologies such as single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have greatly advanced the development of life sciences and clinical medicine. These technologies have revealed cellular heterogeneity and brought novel insights into major fields such as disease, development, and immunity. Howe...

Unveiling a Novel Cancer Hallmark by Evaluation of Neural Infiltration in Cancer

Medicine, Oncology, Life Sciences, Cell Biology, Immunology, Genetics,
neural infiltration   cancer hallmark   tumor microenvironment   single-cell RNA sequencing   immunotherapy  

Cancer, as a major global public health challenge, is characterized by complex mechanisms underlying its onset and progression. For a long time, processes within the tumor microenvironment (TME)—such as immunity, inflammation, and angiogenesis—have been extensively studied and considered key determinants of tumor biological behavior. In recent year...

Exploring Diverse Approaches for Predicting Interferon-Gamma Release: Utilizing MHC Class II and Peptide Sequences

Information Science, Computer Science, Artificial Intelligence, Life Sciences, Biochemistry, Bioengineering, Immunology,
machine learning   T-cell response   immunology   predictive modeling   interferon-gamma   MHC class II   peptide sequences  

Academic Background and Research Significance In recent decades, therapeutic proteins have gained prominence as a research focus in the biopharmaceutical industry due to their huge potential in medicine. With their high targeting ability, therapeutic protein drugs are considered to offer solutions for many acute or chronic diseases (such as certain...