Academic Background RNA isoforms play a critical role in gene expression regulation. On average, human protein-coding genes contain over eight RNA isoforms, leading to nearly four distinct protein-coding sequences. Traditional short-read sequencing technologies, due to their limitations in read length, cannot accurately assemble and quantify RNA is...

Transcriptomics Technology Tracking-Seq Reveals Off-Target Effect Heterogeneity Mediated by CRISPR-Cas9 Research Background With the rapid development of genome editing technologies, the CRISPR-Cas9 system has been widely used in biomedical research due to its high efficiency and ease of operation. However, a significant issue with the CRISPR-Cas9 ...

Major Breakthrough in Research for Autoimmune Neuroinflammation Treatment Background Introduction Steroid resistance is a significant challenge in the treatment of many inflammatory diseases, particularly autoimmune neuroinflammation. In these diseases, TH17 cells (T helper 17 cells) are widely regarded as key contributors to steroid resistance; ho...

Inducing Immune Tolerance through Peripheral Clonal Deletion Targeting PD-1 Expressing Cells Background Introduction The establishment of T-cell receptor (TCR) repertoire through thymic negative selection is a key process for achieving self-tolerance and acquired tolerance post-organ transplantation. However, it remains unclear whether the specific...

Academic News Report: The Temporal and Spatial Bias in β-arrestin Signal Transduction Mediated by G Protein-Coupled Receptor Endocytosis Research Background Among cell surface receptor families, G protein-coupled receptors (GPCRs) constitute the largest family. Upon activation by ligands, they interact with various signaling proteins to trigger int...