Overexpression of ZIC1 Transcription Factor in Segmental Bone Defects Promotes Brown Adipogenic and Osteogenic Differentiation

Medicine, Orthopedics, Basic Medical Sciences, Life Sciences, Cell Biology, Biochemistry, Bioengineering,
ZIC1 transcription factor   osteogenic differentiation   brown adipogenesis   segmental bone defect   stem cells   human adipose progenitor cells   hedgehog signaling  

1. Academic Background and Research Significance Human bone tissue possesses a certain degree of self-healing capability. However, after suffering from severe trauma, tumor resection, infection, or congenital deformity, leading to large-volume bone defects, this regenerative ability becomes limited. The so-called “critical size bone defect” refers ...

METTL3 Promotes Osteogenesis by Regulating N6-Methyladenosine-Dependent Primary Processing of hsa-mir-4526

Medicine, Orthopedics, Basic Medical Sciences, Oral Sciences, Life Sciences, Cell Biology, Biochemistry, Bioengineering,
METTL3   osteogenesis   N6-methyladenosine   miRNA processing   hsa-mir-4526   adipose stem cells   bone repair  

New Mechanism of m6A Methylation Promoting Osteogenic Differentiation of Adipose-Derived Stem Cells Revealed — Study Based on METTL3-Mediated Primary microRNA Splicing Regulation of hsa-mir-4526 1. Academic Background and Research Motivation Bone tissue engineering has emerged as a cutting-edge interdisciplinary research field due to rapid advances...

PDGFR Marks Distinct Mesenchymal and Pericyte Populations Within the Periosteum With Overlapping Cellular Features

Medicine, Orthopedics, Basic Medical Sciences, Life Sciences, Cell Biology, Biochemistry, Bioengineering,
periosteum   PDGFRβ   mesenchymal progenitor cells   pericytes   bone regeneration   single-cell RNA sequencing  

New Developments in Bone Regeneration: The Discovery of PDGFRβ-marked Distinct Mesenchymal and Pericyte Subpopulations in the Periosteum — Interpretation of “pdgfr marks distinct mesenchymal and pericyte populations within the periosteum with overlapping cellular features” 1. Research Background and Scientific Question The periosteum, a thin vascul...

CRISPR-dCas9 Activation of TSG-6 in MSCs Modulates the Cargo of MSC-Derived Extracellular Vesicles and Attenuates Inflammatory Responses in Human Intervertebral Disc Cells In Vitro

Medicine, Rheumatology and Immunology, Orthopedics, Life Sciences, Cell Biology, Immunology,
CRISPR-dCas9   TSG-6   Mesenchymal Stem Cells   Extracellular Vesicles   Intervertebral Disc Cells   Inflammatory Responses   Genomic Engineering  

Intervertebral Disc Degeneration (IVDD) is one of the leading causes of low back pain worldwide, significantly impacting patients’ quality of life. IVDD is often accompanied by inflammatory responses, leading to the degradation of the extracellular matrix (ECM) and structural damage. Although cell therapies, such as mesenchymal stem cells (MSCs), h...

Assessing the Effects of Dasatinib on Mesenchymal Stem/Stromal Cells

Medicine, Orthopedics, Life Sciences, Cell Biology, Bioengineering,
Mesenchymal stem cells   Senescence   Senolytics   Dasatinib   Cell therapy  

Academic Background Mesenchymal Stem/Stromal Cells (MSCs) are a type of multipotent stem cells widely used in regenerative medicine and cell therapy. However, MSCs undergo progressive aging (senescence) during in vitro culture, leading to a decline in their proliferation and differentiation capabilities, which poses a significant obstacle to their ...